› 5d0o

X-ray diffraction
2.9Å resolution

BamABCDE complex, outer membrane beta barrel assembly machinery entire complex

Released:
DOI: 10.2210/pdb5d0o/pdb
PDB entry 5d0o coloured by chain, front view.
PDB entry 5d0o coloured by chain, side view.
PDB entry 5d0o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of outer membrane protein insertion by the BAM complex.
Gu Y, Li H, Dong H, Zeng Y, Zhang Z, Paterson NG, Stansfeld PJ, Wang Z, Zhang Y, Wang W, Dong C
Nature 531 64-9 (2016)
PMID: 26901871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-141767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 392 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Structure domains: Tetratricopeptide repeat domain
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Structure domains: Beta-lactamase Inhibitory Protein; Chain:B, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 116.689Å b: 116.689Å c: 435.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.276 0.304
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Protein folding in the cell envelope of Escherichia coli.
De Geyter et al. (2016)
20 more

45 mentions without citation

The β-barrel assembly machinery in motion.
Noinaj et al. (2017)
44 more