› 5d0y

X-ray diffraction
3.01Å resolution

Substrate bound S-component of folate ECF transporter

Released:
DOI: 10.2210/pdb5d0y/pdb
PDB entry 5d0y coloured by chain, front view.
PDB entry 5d0y coloured by chain, side view.
PDB entry 5d0y coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Primary publication:
Structural insight in the toppling mechanism of an energy-coupling factor transporter.
Swier LJ, Guskov A, Slotboom DJ
Nat Commun 7 11072 (2016)
PMID: 27026363

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved hypothetical membrane protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.52 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Expression system: Lactococcus lactis
UniProt:
  • Canonical: Q1G930 (Residues: 1-176; Coverage: 100%)
Gene name: Ldb1624
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

1 bound ligand:
Ligand FOL 2 x FOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 108.91Å b: 77.54Å c: 89.45Å
α: 90° β: 116.36° γ: 90°
R-values:
R R work R free
0.238 0.235 0.286
Expression system: Lactococcus lactis

Quick links

Citations

4 review citations

Mechanism of Action of ABC Importers: Conservation, Divergence, and Physiological Adaptations.
Lewinson et al. (2017)
3 more

4 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
3 more