› 5d4o

X-ray diffraction
1.8Å resolution

Structure of CPII, a nitrogen regulatory PII-like protein from Thiomonas intermedia K12, bound to ADP, AMP and bicarbonate.

Released:
DOI: 10.2210/pdb5d4o/pdb
PDB entry 5d4o coloured by chain, front view.
PDB entry 5d4o coloured by chain, side view.
PDB entry 5d4o coloured by chain, top view.
Source organism: Thiomonas intermedia K12
Primary publication:
A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
Wheatley NM, Eden KD, Ngo J, Rosinski JS, Sawaya MR, Cascio D, Collazo M, Hoveida H, Hubbell WL, Yeates TO
J Mol Biol 428 4013-4030 (2016)
PMID: 27464895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 108 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thiomonas intermedia K12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5X329 (Residues: 1-108; Coverage: 100%)
Gene name: Tint_0114
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand BCT 2 x BCT
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 51.44Å b: 75.45Å c: 80.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

An overview on the recently discovered iota-carbonic anhydrases.
Nocentini et al. (2021)
4 other articles

1 mention without citation

A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
Wheatley et al. (2016)