› 5d5d

X-ray diffraction
2.4Å resolution

In meso in situ serial X-ray crystallography structure of AlgE at 100 K

Released:
DOI: 10.2210/pdb5d5d/pdb
PDB entry 5d5d coloured by chain, front view.
PDB entry 5d5d coloured by chain, side view.
PDB entry 5d5d coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Struct Biol 72 93-112 (2016)
PMID: 26894538

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate production protein AlgE Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 54.48 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P18895 (Residues: 1-490; Coverage: 100%)
Gene names: PA3544, alg76, algE
Sequence domains: Alginate export
Structure domains: Porin

Ligands and Environments

6 bound ligands:
Ligand NA 2 x NA
Ligand CA 1 x CA
Ligand LDA 7 x LDA
Ligand PE5 5 x PE5
Ligand 78M 2 x 78M
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 46.33Å b: 66.35Å c: 176.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.26
Expression system: Escherichia coli

Quick links

Citations

4 review citations

G Protein-Coupled Receptors in Asthma Therapy: Pharmacology and Drug Action.
Wendell et al. (2020)
3 more

1 mention without citation

In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang et al. (2016)