› 5d6d

X-ray diffraction
3.13Å resolution

Crystal structure of GASDALIE IgG1 Fc in complex with FcgRIIIa

Released:
DOI: 10.2210/pdb5d6d/pdb
PDB entry 5d6d coloured by chain, front view.
PDB entry 5d6d coloured by chain, side view.
PDB entry 5d6d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of GASDALIE Fc bound to the activating Fc receptor FcγRIIIa.
Ahmed AA, Keremane SR, Vielmetter J, Bjorkman PJ
J Struct Biol 194 78-89 (2016)
PMID: 26850169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134854 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 108-328; Coverage: 55%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-A Chain: C
Molecule details ›
Chain: C
Length: 240 amino acids
Theoretical weight: 27.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08637 (Residues: 14-205; Coverage: 79%)
Gene names: CD16A, FCG3, FCGR3, FCGR3A, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
2 bound ligands:
Ligand FUC 1 x FUC
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 74.38Å b: 94.5Å c: 108.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.271 0.296
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Conceptual Approaches to Modulating Antibody Effector Functions and Circulation Half-Life.
Saunders KO. (2019)
13 more

3 mentions without citation

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.
Elledge et al. (2021)
2 more