5d6g

X-ray diffraction
3.3Å resolution

CRYSTAL STRUCTURE OF FRAGMENT OF RIBOSOMAL PROTEIN P0 IN COMPLEX WITH 74NT 23S RNA FROM METHANOCOCCUS JANNASCHII

Released:
Entry authors: Gabdulkhakov AG, Mitroshin IV, Garber MB

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-156931 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL10 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.35 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54049 (Residues: 9-221; Coverage: 63%)
Gene names: MJ0509, rpl10, rplP0
Sequence domains:
Structure domains:
23S ribosomal RNA Chain: 0
Molecule details ›
Chain: 0
Length: 74 nucleotides
Theoretical weight: 23.93 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
Sequence domains: Archaeal large subunit ribosomal RNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I222
Unit cell:
a: 104.23Å b: 108.31Å c: 112.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.298
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli