› 5d7b

X-ray diffraction
3.2Å resolution

Trigonal Crystal Structure of an acetylester hydrolase from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb5d7b/pdb
PDB entry 5d7b coloured by chain, front view.
PDB entry 5d7b coloured by chain, side view.
PDB entry 5d7b coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
A novel esterase subfamily with α/β-hydrolase fold suggested by structures of two bacterial enzymes homologous to L-homoserine O-acetyl transferases.
Tölzer C, Pal S, Watzlawick H, Altenbuchner J, Niefind K
FEBS Lett 590 174-84 (2016)
PMID: 26787467

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable esterase Cgl0839 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38.87 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NS43 (Residues: 1-349; Coverage: 100%)
Gene name: Cgl0839
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 15 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 143.393Å b: 143.393Å c: 197.783Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.201
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A Novel Subfamily Esterase with a Homoserine Transacetylase-like Fold but No Transferase Activity.
Li et al. (2017)
1 more