› 5d7s

X-ray diffraction
1.88Å resolution

Crystal structure of MOR04357, a neutralizing anti-human GM-CSF antibody Fab fragment

Released:
DOI: 10.2210/pdb5d7s/pdb
Model geometry
Fit model/data
PDB entry 5d7s coloured by chain, front view.
PDB entry 5d7s coloured by chain, side view.
PDB entry 5d7s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of in vitro affinity maturation and functional evolution of a neutralizing anti-human GM-CSF antibody.
Eylenstein R, Weinfurtner D, Härtle S, Strohner R, Böttcher J, Augustin M, Ostendorp R, Steidl S
MAbs 8 176-86 (2016)
PMID: 26406987

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213705 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunglobulin G1 Fab fragment, light chain Chain: L
Molecule details ›
Chain: L
Length: 209 amino acids
Theoretical weight: 22.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunglobulin G1 Fab fragment, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand BUD 2 x BUD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 41.823Å b: 66.922Å c: 156.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.254
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Neutralizing GM-CSF autoantibodies in pulmonary alveolar proteinosis, cryptococcal meningitis and severe nocardiosis.
Salvator et al. (2022)
4 more

4 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
3 more