› 5d8c

X-ray diffraction
2.25Å resolution

Crystal structure of HiNmlR, a MerR family regulator lacking the sensor domain, bound to promoter DNA

Released:
DOI: 10.2210/pdb5d8c/pdb
PDB entry 5d8c coloured by chain, front view.
PDB entry 5d8c coloured by chain, side view.
PDB entry 5d8c coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Structural basis of thiol-based regulation of formaldehyde detoxification in H. influenzae by a MerR regulator with no sensor region.
Couñago RM, Chen NH, Chang CW, Djoko KY, McEwan AG, Kobe B
Nucleic Acids Res 44 6981-93 (2016)
PMID: 27307602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118543 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Uncharacterized HTH-type transcriptional regulator HI_0186 Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.69 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44558 (Residues: 1-135; Coverage: 100%)
Gene name: HI_0186
Sequence domains: MerR family regulatory protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
DNA (5'-D(*CP*TP*TP*AP*GP*AP*GP*TP*TP*CP*AP*CP*TP*CP*TP*AP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Not provided
DNA (5'-D(*CP*TP*TP*AP*GP*AP*GP*TP*GP*AP*AP*CP*TP*CP*TP*AP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 56.03Å b: 111.82Å c: 103.17Å
α: 90° β: 102.1° γ: 90°
R-values:
R R work R free
0.21 0.208 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
2 more

3 mentions without citation

Structural basis of thiol-based regulation of formaldehyde detoxification in H. influenzae by a MerR regulator with no sensor region.
Couñago et al. (2016)
2 more