› 5d91

X-ray diffraction
2.5Å resolution

Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum

Released:
DOI: 10.2210/pdb5d91/pdb
PDB entry 5d91 coloured by chain, front view.
PDB entry 5d91 coloured by chain, side view.
PDB entry 5d91 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for phosphatidylinositol-phosphate biosynthesis.
Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F
Nat Commun 6 8505 (2015)
PMID: 26510127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-alcohol phosphatidyltransferase AF-2299-like N-terminal domain-containing protein; Phosphatidylinositol phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.95 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: O27985 (Residues: 1-135, 161-161, 168-169, 223-223, 225-225, 227-227; Coverage: 41%)
  • Canonical: A9WSF5 (Residues: 7-14, 16-21, 24-74, 76-76, 78-78, 80-205; Coverage: 94%)
Gene names: AF_2299, RSal33209_2010
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand 8K6 35 x 8K6
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 48.632Å b: 94.069Å c: 103.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.247
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Lipid topogenesis--35years on.
Chauhan et al. (2016)
4 more

3 mentions without citation

Structural basis for catalysis at the membrane-water interface.
Dufrisne et al. (2017)
2 more