› 5d9g

X-ray diffraction
2.15Å resolution

Crystal structure of TIPRL, TOR signaling pathway regulator-like, in complex with peptide

Released:
DOI: 10.2210/pdb5d9g/pdb
PDB entry 5d9g coloured by chain, front view.
PDB entry 5d9g coloured by chain, side view.
PDB entry 5d9g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human Tip41 orthologue, TIPRL, reveals a novel fold and a binding site for the PP2Ac C-terminus.
Scorsato V, Lima TB, Righetto GL, Zanchin NI, Brandão-Neto J, Sandy J, Pereira HD, Ferrari ÁJ, Gozzo FC, Smetana JH, Aparicio R
Sci Rep 6 30813 (2016)
PMID: 27489114

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131094 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TIP41-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 28.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75663 (Residues: 16-256; Coverage: 89%)
Gene name: TIPRL
Sequence domains: TIP41-like family
oligo peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 813 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand PO4 2 x PO4
Ligand NI 1 x NI
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P65
Unit cell:
a: 145.307Å b: 145.307Å c: 95.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.198
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biased holoenzyme assembly of protein phosphatase 2A (PP2A): From cancer to small molecules.
Haanen et al. (2022)
1 more

3 mentions without citation

Crystal structure of the human Tip41 orthologue, TIPRL, reveals a novel fold and a binding site for the PP2Ac C-terminus.
Scorsato et al. (2016)
2 more