› 5da0

X-ray diffraction
3.2Å resolution

Structure of the the SLC26 transporter SLC26Dg in complex with a nanobody

Released:
DOI: 10.2210/pdb5da0/pdb
PDB entry 5da0 coloured by chain, front view.
PDB entry 5da0 coloured by chain, side view.
PDB entry 5da0 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.
Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R
Nat Struct Mol Biol 22 803-8 (2015)
PMID: 26367249

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212069 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
STAS domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 505 amino acids
Theoretical weight: 54.15 KDa
Source organism: Deinococcus geothermalis DSM 11300
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1J2S8 (Residues: 1-499; Coverage: 100%)
Gene name: Dgeo_2773
Sequence domains:
Nanobody Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 13.22 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand DMU 2 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 127.65Å b: 117.28Å c: 84.36Å
α: 90° β: 100.09° γ: 90°
R-values:
R R work R free
0.261 0.258 0.308
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
22 more

14 mentions without citation

Novel Roles for Chloride Channels, Exchangers, and Regulators in Chronic Inflammatory Airway Diseases.
Sala-Rabanal et al. (2015)
13 more