› 5db5

X-ray diffraction
2.75Å resolution

Crystal structure of PLP-bound E. coli SufS (cysteine persulfide intermediate) in space group P21

Released:
DOI: 10.2210/pdb5db5/pdb
PDB entry 5db5 coloured by chain, front view.
PDB entry 5db5 coloured by chain, side view.
PDB entry 5db5 coloured by chain, top view.
Source organism: Escherichia coli DH5[alpha]
Entry authors: Arbing MA, Shin A, Koo CW, Medrano-Soto A, Eisenberg D

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 44.6 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand EDO 2 x EDO
Ligand CYS 1 x CYS
Ligand CIT 1 x CIT
1 modified residue:
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 59.68Å b: 114.15Å c: 63.11Å
α: 90° β: 116.21° γ: 90°
R-values:
R R work R free
0.168 0.165 0.217
Expression system: Escherichia coli BL21(DE3)

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