› 5dcf

X-ray diffraction
2.3Å resolution

C-terminal domain of XerD recombinase in complex with gamma domain of FtsK

Released:
DOI: 10.2210/pdb5dcf/pdb
PDB entry 5dcf coloured by chain, front view.
PDB entry 5dcf coloured by chain, side view.
PDB entry 5dcf coloured by chain, top view.
Source organism: Escherichia coli CFT073
Primary publication:
Activation of Xer-recombination at dif: structural basis of the FtsKγ-XerD interaction.
Keller AN, Xin Y, Boer S, Reinhardt J, Baker R, Arciszewska LK, Lewis PJ, Sherratt DJ, Löwe J, Grainge I
Sci Rep 6 33357 (2016)
PMID: 27708355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine recombinase XerC Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.73 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8P6 (Residues: 112-129, 130-195, 196-197, 198-236, 237-294; Coverage: 61%)
Gene names: JW3784, b3811, xerC
Sequence domains: Phage integrase family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P65
Unit cell:
a: 83.451Å b: 83.451Å c: 88.672Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Xer Site Specific Recombination: Double and Single Recombinase Systems.
Castillo et al. (2017)
2 more

3 mentions without citation

Characterization and induction of prophages in human gut-associated Bifidobacterium hosts.
Mavrich et al. (2018)
2 more