› 5dcq

X-ray diffraction
1.83Å resolution

Crystal structure of bacterial adhesin, FNE from Streptococcus equi spp. equi.

Released:
DOI: 10.2210/pdb5dcq/pdb
PDB entry 5dcq coloured by chain, front view.
PDB entry 5dcq coloured by chain, side view.
PDB entry 5dcq coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analysis of the fibronectin-binding protein FNE from Streptococcus equi spp. equi.
Tiouajni M, Durand D, Blondeau K, Graille M, Urvoas A, Valerio-Lepiniec M, Guellouz A, Aumont-Nicaise M, Minard P, van Tilbeurgh H
FEBS J 281 5513-31 (2014)
PMID: 25290767

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165553 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
artificial repeat proteins (alphaREP3) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 170 amino acids
Theoretical weight: 18.68 KDa
Source organism: synthetic construct
Expression system: Escherichia coli M15
Thioester domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 271 amino acids
Theoretical weight: 30.7 KDa
Source organism: Streptococcus equi subsp. equi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q93ED6 (Residues: 35-298; Coverage: 89%)
Gene name: fne
Sequence domains: Thioester domain

Ligands and Environments

1 bound ligand:
Ligand FMT 12 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P61
Unit cell:
a: 113.688Å b: 113.688Å c: 152.623Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.202
Expression systems:
  • Escherichia coli M15
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 review citations

Collagen-like proteins of pathogenic streptococci.
Lukomski et al. (2017)
1 more