› 5dfk

X-ray diffraction
2.4Å resolution

Crystal Structure of the Escherichia coli Common Pilus Chaperone, EcpB

Released:
DOI: 10.2210/pdb5dfk/pdb
PDB entry 5dfk coloured by chain, front view.
PDB entry 5dfk coloured by chain, side view.
PDB entry 5dfk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Insight into Archaic and Alternative Chaperone-Usher Pathways Reveals a Novel Mechanism of Pilus Biogenesis.
Pakharukova N, Garnett JA, Tuittila M, Paavilainen S, Diallo M, Xu Y, Matthews SJ, Zavialov AV
PLoS Pathog 11 e1005269 (2015)
PMID: 26587649

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable fimbrial chaperone EcpB Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli CFT073
UniProt:
  • Canonical: P77188 (Residues: 6-6, 21-222; Coverage: 93%)
Gene names: JW0286, b0292, ecpB, matC, yagY
Sequence domains: EcpB C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 62.65Å b: 62.65Å c: 121.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.238
Expression system: Escherichia coli CFT073

Quick links

Citations

5 review citations

Classical chaperone-usher (CU) adhesive fimbriome: uropathogenic Escherichia coli (UPEC) and urinary tract infections (UTIs).
Behzadi P. (2020)
4 more

2 mentions without citation

Structural Insight into Archaic and Alternative Chaperone-Usher Pathways Reveals a Novel Mechanism of Pilus Biogenesis.
Pakharukova et al. (2015)
1 more