› 5dfm

X-ray diffraction
2.3Å resolution

Structure of Tetrahymena telomerase p19 fused to MBP

Released:
DOI: 10.2210/pdb5dfm/pdb
PDB entry 5dfm coloured by chain, front view.
PDB entry 5dfm coloured by chain, side view.
PDB entry 5dfm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.
Jiang J, Chan H, Cash DD, Miracco EJ, Ogorzalek Loo RR, Upton HE, Cascio D, O'Brien Johnson R, Collins K, Loo JA, Zhou ZH, Feigon J
Science 350 aab4070 (2015)
PMID: 26472759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 534 amino acids
Theoretical weight: 59.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 15 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 150.6Å b: 150.6Å c: 150.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.175 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Regulation of human telomerase in homeostasis and disease.
Roake et al. (2020)
38 more

2 mentions without citation

Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.
Jiang et al. (2015)
1 more