› 5dfn

X-ray diffraction
2.38Å resolution

Structure of Tetrahymena Telomerase P45 C-terminal domain

Released:
DOI: 10.2210/pdb5dfn/pdb
PDB entry 5dfn coloured by chain, front view.
PDB entry 5dfn coloured by chain, side view.
PDB entry 5dfn coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.
Jiang J, Chan H, Cash DD, Miracco EJ, Ogorzalek Loo RR, Upton HE, Cascio D, O'Brien Johnson R, Collins K, Loo JA, Zhou ZH, Feigon J
Science 350 aab4070 (2015)
PMID: 26472759

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Telomerase-associated protein of 45 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 25.21 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6JXI5 (Residues: 161-373; Coverage: 57%)
Gene names: TAP45, TTHERM_00083360

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: F222
Unit cell:
a: 88.03Å b: 124.62Å c: 173.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.207 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

Regulation of human telomerase in homeostasis and disease.
Roake et al. (2020)
35 more

4 mentions without citation

Progress in Human and Tetrahymena Telomerase Structure Determination.
Chan et al. (2017)
3 more