› 5dgk

X-ray diffraction
2.9Å resolution

SCCmec type IV Cch - active helicase

Released:
DOI: 10.2210/pdb5dgk/pdb
PDB entry 5dgk coloured by chain, front view.
PDB entry 5dgk coloured by chain, side view.
PDB entry 5dgk coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Mir-Sanchis I, Roman CA, Misiura A, Pigli YZ, Boyle-Vavra S, Rice PA
Nat Struct Mol Biol 23 891-898 (2016)
PMID: 27571176

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SCCmec staphylococcal cassette region, isolate CMFT454 Chains: A, B
Molecule details ›
Chains: A, B
Length: 603 amino acids
Theoretical weight: 68.31 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q0WXP6 (Residues: 1-595; Coverage: 100%)
Gene names: EQ90_14515, GO814_14830, GO942_10655, R114_17, R92_17
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 110.14Å b: 110.14Å c: 302.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.261
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 review citations

Mobile Genetic Elements Associated with Antimicrobial Resistance.
Partridge et al. (2018)
3 more

1 mention without citation

Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Mir-Sanchis et al. (2016)