› 5dhe

X-ray diffraction
1.6Å resolution

Crystal structure of ChBD3 from Thermococcus kodakarensis KOD1

Released:
DOI: 10.2210/pdb5dhe/pdb
Model geometry
Fit model/data
PDB entry 5dhe coloured by chain, front view.
PDB entry 5dhe coloured by chain, side view.
PDB entry 5dhe coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Crystal structures of chitin binding domains of chitinase from Thermococcus kodakarensis KOD1.
Hanazono Y, Takeda K, Niwa S, Hibi M, Takahashi N, Kanai T, Atomi H, Miki K
FEBS Lett 590 298-304 (2016)
PMID: 26823175

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 11.01 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UWR7 (Residues: 764-863; Coverage: 8%)
Gene names: Pk-chiA, TK1765
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 49.161Å b: 44.669Å c: 51.712Å
α: 90° β: 114.88° γ: 90°
R-values:
R R work R free
0.194 0.194 0.209
Expression system: Escherichia coli

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Citations

3 review citations

An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.
Rashid et al. (2020)
2 more