› 5dhz

X-ray diffraction
4.3Å resolution

HIV-1 Rev NTD dimers with variable crossing angles

Released:
DOI: 10.2210/pdb5dhz/pdb
PDB entry 5dhz coloured by chain, front view.
PDB entry 5dhz coloured by chain, side view.
PDB entry 5dhz coloured by chain, top view.
Source organisms:
Primary publication:
The Structure of HIV-1 Rev Filaments Suggests a Bilateral Model for Rev-RRE Assembly.
DiMattia MA, Watts NR, Cheng N, Huang R, Heymann JB, Grimes JM, Wingfield PT, Stuart DI, Steven AC
Structure 24 1068-80 (2016)
PMID: 27265851

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209110 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Anti-Rev Antibody Fab single-chain variable fragment, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 117 amino acids
Theoretical weight: 12.58 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
Anti-Rev Antibody Fab single-chain variable fragment, light chain Chain: L
Molecule details ›
Chain: L
Length: 110 amino acids
Theoretical weight: 11.66 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
Protein Rev Chain: M
Molecule details ›
Chain: M
Length: 65 amino acids
Theoretical weight: 7.75 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P04616 (Residues: 1-65; Coverage: 56%)
Gene name: rev
Sequence domains: REV protein (anti-repression trans-activator protein)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3212
Unit cell:
a: 48.54Å b: 48.54Å c: 264.482Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.328 0.328 0.324
Expression system: Escherichia coli

Quick links

Citations

2 review citations

CAR-T cell potency: from structural elements to vector backbone components.
Mazinani et al. (2022)
1 more

2 mentions without citation

The Structure of HIV-1 Rev Filaments Suggests a Bilateral Model for Rev-RRE Assembly.
DiMattia et al. (2016)
1 more