› 5di5

X-ray diffraction
2.28Å resolution

beta1 t801 loop variant in P3221

Released:
DOI: 10.2210/pdb5di5/pdb
PDB entry 5di5 coloured by chain, front view.
PDB entry 5di5 coloured by chain, side view.
PDB entry 5di5 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.
MacDonald JT, Kabasakal BV, Godding D, Kraatz S, Henderson L, Barber J, Freemont PS, Murray JW
Proc Natl Acad Sci U S A 113 10346-51 (2016)
PMID: 27573845

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta1 t801 loop variant Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 25.48 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3221
Unit cell:
a: 123.46Å b: 123.46Å c: 47.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Computational design of structured loops for new protein functions.
Kundert et al. (2019)
7 more

1 mention without citation

Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.
MacDonald et al. (2016)