› 5dkm

X-ray diffraction
0.92Å resolution

S. erythraea trypsin Michaelis-Menten complex

Released:
DOI: 10.2210/pdb5dkm/pdb
Model geometry
Fit model/data
PDB entry 5dkm coloured by chain, front view.
PDB entry 5dkm coloured by chain, side view.
PDB entry 5dkm coloured by chain, top view.
Source organism: Saccharopolyspora erythraea
Entry authors: Blankenship E, Lodowski DT

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase S1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 23.66 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q54137 (Residues: 43-272; Coverage: 92%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 42.072Å b: 47.441Å c: 53.471Å
α: 90° β: 106.1° γ: 90°
R-values:
R R work R free
0.12 0.119 0.13
Expression system: Escherichia coli BL21(DE3)

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