› 5dok

X-ray diffraction
2.3Å resolution

Crystal structure of Tetrahymena p45C

Released:
DOI: 10.2210/pdb5dok/pdb
PDB entry 5dok coloured by chain, front view.
PDB entry 5dok coloured by chain, side view.
PDB entry 5dok coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.
Wan B, Tang T, Upton H, Shuai J, Zhou Y, Li S, Chen J, Brunzelle JS, Zeng Z, Collins K, Wu J, Lei M
Nat Struct Mol Biol 22 1023-6 (2015)
PMID: 26551074

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Telomerase-associated protein of 45 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.99 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6JXI5 (Residues: 170-373; Coverage: 55%)
Gene names: TAP45, TTHERM_00083360

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 75.999Å b: 89.051Å c: 75.888Å
α: 90° β: 108.64° γ: 90°
R-values:
R R work R free
0.226 0.223 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Telomerase Mechanism of Telomere Synthesis.
Wu et al. (2017)
11 more

1 mention without citation

The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.
Wan et al. (2015)