› 5du7

X-ray diffraction
1.79Å resolution

Crystal structure of ldtMt2 at 1.79 Angstrom resolution

Released:
DOI: 10.2210/pdb5du7/pdb
PDB entry 5du7 coloured by chain, front view.
PDB entry 5du7 coloured by chain, side view.
PDB entry 5du7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Non-classical transpeptidases yield insight into new antibacterials.
Kumar P, Kaushik A, Lloyd EP, Li SG, Mattoo R, Ammerman NC, Bell DT, Perryman AL, Zandi TA, Ekins S, Ginell SL, Townsend CA, Freundlich JS, Lamichhane G
Nat Chem Biol 13 54-61 (2017)
PMID: 27820797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-125201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L,D-transpeptidase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 39.53 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6Y9J2 (Residues: 42-408; Coverage: 98%)
Gene names: P425_02624, RVBD_2518c, Rv2518c, ldtB, lppS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 61.644Å b: 75.534Å c: 94.143Å
α: 89.04° β: 89.96° γ: 92.76°
R-values:
R R work R free
0.172 0.17 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mycobacterium abscessus and β-Lactams: Emerging Insights and Potential Opportunities.
Story-Roller et al. (2018)
7 more

6 mentions without citation

Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.
Vermassen et al. (2019)
5 more