› 5dun

X-ray diffraction
2.64Å resolution

The crystal structure of OMe substituted twister ribozyme

Released:
DOI: 10.2210/pdb5dun/pdb
PDB entry 5dun coloured by chain, front view.
PDB entry 5dun coloured by chain, side view.
PDB entry 5dun coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A Mini-Twister Variant and Impact of Residues/Cations on the Phosphodiester Cleavage of this Ribozyme Class.
Košutić M, Neuner S, Ren A, Flür S, Wunderlich C, Mairhofer E, Vušurović N, Seikowski J, Breuker K, Höbartner C, Patel DJ, Kreutz C, Micura R
Angew Chem Int Ed Engl 54 15128-15133 (2015)
PMID: 26473980

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196920 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (54-MER) Chain: A
Molecule details ›
Chain: A
Length: 54 nucleotides
Theoretical weight: 17.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand K 1 x K
1 modified residue:
Ligand OMU 1 x OMU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 46.819Å b: 46.819Å c: 143.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.252
Expression system: Not provided

Quick links

Citations

4 review citations

Structure-based mechanistic insights into catalysis by small self-cleaving ribozymes.
Ren et al. (2017)
3 more

10 mentions without citation

Unwinding the twister ribozyme: from structure to mechanism.
Gebetsberger et al. (2017)
9 more