› 5dzr

X-ray diffraction
3.16Å resolution

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

Released:
DOI: 10.2210/pdb5dzr/pdb
PDB entry 5dzr coloured by chain, front view.
PDB entry 5dzr coloured by chain, side view.
PDB entry 5dzr coloured by chain, top view.
Source organism: Neurospora crassa OR74A
Primary publication:
Structure of the frequency-interacting RNA helicase: a protein interaction hub for the circadian clock.
Conrad KS, Hurley JM, Widom J, Ringelberg CS, Loros JJ, Dunlap JC, Crane BR
EMBO J 35 1707-19 (2016)
PMID: 27340124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FRQ-interacting RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 993 amino acids
Theoretical weight: 112.83 KDa
Source organism: Neurospora crassa OR74A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1K502 (Residues: 114-1106; Coverage: 90%)
Gene names: NCU03363, frh
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 76.797Å b: 107.749Å c: 125.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.264 0.325
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Functions and regulation of the Brr2 RNA helicase during splicing.
Absmeier et al. (2016)
3 more

2 mentions without citation

Mtr4 RNA helicase structures and interactions.
Olsen et al. (2021)
1 more