› 5e02

X-ray diffraction
3.8Å resolution

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

Released:
DOI: 10.2210/pdb5e02/pdb
PDB entry 5e02 coloured by chain, front view.
PDB entry 5e02 coloured by chain, side view.
PDB entry 5e02 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the frequency-interacting RNA helicase: a protein interaction hub for the circadian clock.
Conrad KS, Hurley JM, Widom J, Ringelberg CS, Loros JJ, Dunlap JC, Crane BR
EMBO J 35 1707-19 (2016)
PMID: 27340124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172914 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
FRQ-interacting RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 1106 amino acids
Theoretical weight: 124.64 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1K502 (Residues: 1-1106; Coverage: 100%)
Gene names: NCU03363, frh
Sequence domains:
RNA (5'-R(*AP*AP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.27 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 76.432Å b: 106.6Å c: 125.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.278 0.321
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Intrinsic disorder is an essential characteristic of components in the conserved circadian circuit.
Pelham et al. (2020)
2 more

4 mentions without citation

Functions and regulation of the Brr2 RNA helicase during splicing.
Absmeier et al. (2016)
3 more