› 5e4y

X-ray diffraction
2.8Å resolution

Orthorhombic structure of the acetyl esterase MekB

Released:
DOI: 10.2210/pdb5e4y/pdb
PDB entry 5e4y coloured by chain, front view.
PDB entry 5e4y coloured by chain, side view.
PDB entry 5e4y coloured by chain, top view.
Source organism: Pseudomonas veronii
Primary publication:
A novel esterase subfamily with α/β-hydrolase fold suggested by structures of two bacterial enzymes homologous to L-homoserine O-acetyl transferases.
Tölzer C, Pal S, Watzlawick H, Altenbuchner J, Niefind K
FEBS Lett 590 174-84 (2016)
PMID: 26787467

Function and Biology Details

Reaction catalysed: 
Ethyl acetate + H(2)O = acetate + ethanol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ethyl acetate hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 39.75 KDa
Source organism: Pseudomonas veronii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0MRG5 (Residues: 2-349; Coverage: 100%)
Gene name: mekB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 58.346Å b: 79.946Å c: 142.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.237
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A Novel Subfamily Esterase with a Homoserine Transacetylase-like Fold but No Transferase Activity.
Li et al. (2017)
1 more