› 5e5p

X-ray diffraction
2.65Å resolution

Wild type I-SmaMI in the space group of C121

Released:
DOI: 10.2210/pdb5e5p/pdb
PDB entry 5e5p coloured by chain, front view.
PDB entry 5e5p coloured by chain, side view.
PDB entry 5e5p coloured by chain, top view.
Source organism: Sordaria macrospora k-hell
Primary publication:
The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
Shen BW, Lambert A, Walker BC, Stoddard BL, Kaiser BK
J Mol Biol 428 206-220 (2016)
PMID: 26705195

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.15 KDa
Source organism: Sordaria macrospora k-hell
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7WD42 (Residues: 114-415; Coverage: 70%)
Gene name: SMAC_12671
Sequence domains: LAGLIDADG endonuclease

Ligands and Environments

2 bound ligands:
Ligand PG0 1 x PG0
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 148.327Å b: 58.23Å c: 42.046Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.179 0.175 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Lambert et al. (2016)
2 more

1 mention without citation

The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
Shen et al. (2016)