› 5e6g

X-ray diffraction
2.09Å resolution

Crystal Structure of De Novo Designed Protein CA01

Released:
DOI: 10.2210/pdb5e6g/pdb
PDB entry 5e6g coloured by chain, front view.
PDB entry 5e6g coloured by chain, side view.
PDB entry 5e6g coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Design of structurally distinct proteins using strategies inspired by evolution.
Jacobs TM, Williams B, Williams T, Xu X, Eletsky A, Federizon JF, Szyperski T, Kuhlman B
Science 352 687-90 (2016)
PMID: 27151863

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-165588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
De novo designed protein CA01 Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.53 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R3
Unit cell:
a: 116.537Å b: 116.537Å c: 57.215Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.234
Expression system: Escherichia coli

Quick links

Citations

18 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
17 more

3 mentions without citation

Design and Engineering of Miniproteins.
Ożga et al. (2022)
2 more