› 5e8g

X-ray diffraction
2.7Å resolution

Crystal structure of the DNA binding domain of human transcription factor FLI1

Released:
DOI: 10.2210/pdb5e8g/pdb
PDB entry 5e8g coloured by chain, front view.
PDB entry 5e8g coloured by chain, side view.
PDB entry 5e8g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Dimerization and DNA Binding of Transcription Factor FLI1.
Hou C, Tsodikov OV
Biochemistry 54 7365-74 (2015)
PMID: 26618620

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Friend leukemia integration 1 transcription factor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 14.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-399; Coverage: 27%)
Gene name: FLI1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand CO 4 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: R3
Unit cell:
a: 140.551Å b: 140.551Å c: 85.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Hematopoietic transcription factor mutations: important players in inherited platelet defects.
Songdej et al. (2017)
1 more

1 mention without citation

Fli-1 Activation through Targeted Promoter Activity Regulation Using a Novel 3', 5'-diprenylated Chalcone Inhibits Growth and Metastasis of Prostate Cancer Cells.
Ma et al. (2020)