› 5e96

X-ray diffraction
2.1Å resolution

Crystal structure of aminoglycoside 6'-acetyltransferase type Ii

Released:
DOI: 10.2210/pdb5e96/pdb
Model geometry
Fit model/data
PDB entry 5e96 coloured by chain, front view.
PDB entry 5e96 coloured by chain, side view.
PDB entry 5e96 coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
Comprehensive characterization of ligand-induced plasticity changes in a dimeric enzyme.
Baettig OM, Shi K, Yachnin BJ, Burk DL, Berghuis AM
OpenAccess logo FEBS J 283 3029-38 (2016)
PMID: 27333541

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.51 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47764 (Residues: 1-180; Coverage: 99%)
Gene name: aac(6')-Ii
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P64
Unit cell:
a: 66.815Å b: 66.815Å c: 104.129Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.256
Expression system: Escherichia coli

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Citations

3 mentions without citation

Overcoming Aminoglycoside Enzymatic Resistance: Design of Novel Antibiotics and Inhibitors.
Zárate et al. (2018)
2 more