› 5ea0

X-ray diffraction
2Å resolution

Structure of the antibody 7968 with human complement factor H-derived peptide

Released:
DOI: 10.2210/pdb5ea0/pdb
PDB entry 5ea0 coloured by chain, front view.
PDB entry 5ea0 coloured by chain, side view.
PDB entry 5ea0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Therapeutic Antibody for Cancer, Derived from Single Human B Cells.
Bushey RT, Moody MA, Nicely NL, Haynes BF, Alam SM, Keir ST, Bentley RC, Roy Choudhury K, Gottlin EB, Campa MJ, Liao HX, Patz EF
Cell Rep 15 1505-1513 (2016)
PMID: 27160908

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229951 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy chain of antibody 7968 Fab fragment Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Light chain of antibody 7968 Fab fragment Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Complement factor H-related protein 2 Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P36980 (Residues: 150-162; Coverage: 5%)
Gene names: CFHL2, CFHR2, FHR2, HFL3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 51.28Å b: 65.547Å c: 71.267Å
α: 90° β: 106.37° γ: 90°
R-values:
R R work R free
0.202 0.196 0.275
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

6 review citations

Development of therapeutic antibodies for the treatment of diseases.
Lu et al. (2020)
5 more

3 mentions without citation

Rapid assessment of oxidation via middle-down LCMS correlates with methionine side-chain solvent-accessible surface area for 121 clinical stage monoclonal antibodies.
Yang et al. (2017)
2 more