› 5ebj

Hybrid

Joint X-ray/neutron structure of reversibly photoswitching chromogenic protein, Dathail

Released:
DOI: 10.2210/pdb5ebj/pdb
PDB entry 5ebj coloured by chain, front view.
PDB entry 5ebj coloured by chain, side view.
PDB entry 5ebj coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Evolution and characterization of a new reversibly photoswitching chromogenic protein, Dathail.
Langan PS, Close DW, Coates L, Rocha RC, Ghosh K, Kiss C, Waldo G, Freyer J, Kovalevsky A, Bradbury AR
J Mol Biol 428 1776-89 (2016)
PMID: 27000644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
photoswitching chromogenic protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.9 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand DOD 92 x DOD
1 modified residue:
Ligand CRQ 1 x CRQ

Experiments and Validation Details

Experimental Method: Neutron Diffraction, X-ray diffraction
Spacegroup: P21212
Unit cell:
a: 77.209Å b: 82.049Å c: 40.889Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.305 0.305 0.344
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
1 more

2 mentions without citation

Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.
Liebschner et al. (2018)
1 more