› 5ebz

X-ray diffraction
4.5Å resolution

Crystal structure of human IKK1

Released:
DOI: 10.2210/pdb5ebz/pdb
PDB entry 5ebz coloured by chain, front view.
PDB entry 5ebz coloured by chain, side view.
PDB entry 5ebz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Activation of IKK1/α.
Polley S, Passos DO, Huang DB, Mulero MC, Mazumder A, Biswas T, Verma IM, Lyumkis D, Ghosh G
Cell Rep 17 1907-1914 (2016)
PMID: 27851956

Function and Biology Details

Reaction catalysed: 
ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Inhibitor of nuclear factor kappa-B kinase subunit alpha Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 655 amino acids
Theoretical weight: 75.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15111 (Residues: 10-660; Coverage: 87%)
Gene names: CHUK, IKKA, TCF16
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: 5LS, GLC, PDX
Carbohydrate polymer
Carbohydrate polymer : NEW Components: 5LS, 5TJ, 5TM, Z4K, 5TK, 5TH
Ligand 5LS 4 x 5LS
Ligand 5TJ 8 x 5TJ
Ligand 5TM 4 x 5TM
Ligand Z4K 4 x Z4K
Ligand 5TK 4 x 5TK
Ligand 5TH 4 x 5TH
1 bound ligand:
Ligand 5TL 12 x 5TL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 174.51Å b: 186.94Å c: 275.83Å
α: 90° β: 98.84° γ: 90°
R-values:
R R work R free
0.238 0.238 0.276
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

NFκB and Kidney Injury.
Song et al. (2019)
3 more

19 mentions without citation

Targeting IKKβ in Cancer: Challenges and Opportunities for the Therapeutic Utilisation of IKKβ Inhibitors.
Prescott et al. (2018)
18 more