› 5ec5

X-ray diffraction
3.1Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysenin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S
Length: 298 amino acids
Theoretical weight: 33.44 KDa
Source organism: Eisenia fetida
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O18423 (Residues: 1-297; Coverage: 100%)
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:
Ligand MBO 22 x MBO
Ligand HG 14 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P43212
Unit cell:
a: 192.994Å b: 192.994Å c: 493.202Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.258
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

13 review citations

Pore formation in regulated cell death.
Flores-Romero et al. (2020)
12 more

10 mentions without citation

Functional and Regulatory Roles of Fold-Switching Proteins.
Kim et al. (2021)
9 more