› 5ee5

X-ray diffraction
2.28Å resolution

Structure of human ARL1 in complex with the DCB domain of BIG1

Released:
DOI: 10.2210/pdb5ee5/pdb
PDB entry 5ee5 coloured by chain, front view.
PDB entry 5ee5 coloured by chain, side view.
PDB entry 5ee5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Galindo A, Soler N, McLaughlin SH, Yu M, Williams RL, Munro S
Cell Rep 16 839-50 (2016)
PMID: 27373159

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154151 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y6D6 (Residues: 1-229; Coverage: 11%)
Gene names: ARFGEF1, ARFGEP1, BIG1
Sequence domains: Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
ADP-ribosylation factor-like protein 1 Chain: B
Molecule details ›
Chain: B
Length: 171 amino acids
Theoretical weight: 19.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40616 (Residues: 15-181; Coverage: 92%)
Gene name: ARL1
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

6 bound ligands:
Ligand NA 14 x NA
Ligand GOL 2 x GOL
Ligand CL 7 x CL
Ligand GTP 1 x GTP
Ligand MG 4 x MG
Ligand ACT 1 x ACT
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 84.166Å b: 50.727Å c: 103.837Å
α: 90° β: 111.98° γ: 90°
R-values:
R R work R free
0.219 0.217 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Extensive GTPase crosstalk regulates Golgi trafficking and maturation.
Thomas et al. (2020)
3 more

2 mentions without citation

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
1 more