› 5ee9

X-ray diffraction
2.75Å resolution

Complex structure of OSYCHF1 with GMP-PNP

Released:
DOI: 10.2210/pdb5ee9/pdb
Model geometry
Fit model/data
PDB entry 5ee9 coloured by chain, front view.
PDB entry 5ee9 coloured by chain, side view.
PDB entry 5ee9 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
ATP binding by the P-loop NTPase OsYchF1 (an unconventional G protein) contributes to biotic but not abiotic stress responses.
Cheung MY, Li X, Miao R, Fong YH, Li KP, Yung YL, Yu MH, Wong KB, Chen Z, Lam HM
Proc Natl Acad Sci U S A 113 2648-53 (2016)
PMID: 26912459

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obg-like ATPase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 44.49 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6Z1J6 (Residues: 1-394; Coverage: 100%)
Gene names: LOC_Os08g09940, OSJNBb0094P23.28, Os08g0199300, OsJ_26368, YCHF1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand K 1 x K
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 70.451Å b: 112.064Å c: 115.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Plant Hormone Signaling Crosstalks between Biotic and Abiotic Stress Responses.
Ku et al. (2018)
4 more

3 mentions without citation

Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein.
Luo et al. (2022)
2 more