› 5eg7

X-ray diffraction
1.4Å resolution

The cap binding site of influenza virus protein PB2 as a drug target

Released:
DOI: 10.2210/pdb5eg7/pdb
PDB entry 5eg7 coloured by chain, front view.
PDB entry 5eg7 coloured by chain, side view.
PDB entry 5eg7 coloured by chain, top view.
Source organism: Influenza A virus (A/swine/Quebec/1257777/2010(H3N2))
Primary publication:
The cap-binding site of influenza virus protein PB2 as a drug target.
Severin C, Rocha de Moura T, Liu Y, Li K, Zheng X, Luo M
Acta Crystallogr D Struct Biol 72 245-53 (2016)
PMID: 26894672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.04 KDa
Source organism: Influenza A virus (A/swine/Quebec/1257777/2010(H3N2))
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G3LZH2 (Residues: 106-207, 215-270; Coverage: 29%)
Gene name: PB2
Sequence domains: Influenza RNA polymerase PB2 CAP binding domain

Ligands and Environments

2 bound ligands:
Ligand MGT 1 x MGT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 45.262Å b: 45.262Å c: 156.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.183
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design.
Stevaert et al. (2016)
3 more

1 mention without citation

High-resolution structure of the Influenza A virus PB2cap binding domain illuminates the changes induced by ligand binding.
Constantinides et al. (2018)