› 5eg8

X-ray diffraction
1.54Å resolution

The cap binding site of influenza virus protein PB2 as a drug target

Released:
DOI: 10.2210/pdb5eg8/pdb
PDB entry 5eg8 coloured by chain, front view.
PDB entry 5eg8 coloured by chain, side view.
PDB entry 5eg8 coloured by chain, top view.
Source organism: Influenza A virus (A/swine/Quebec/1257777/2010(H3N2))
Primary publication:
The cap-binding site of influenza virus protein PB2 as a drug target.
Severin C, Rocha de Moura T, Liu Y, Li K, Zheng X, Luo M
Acta Crystallogr D Struct Biol 72 245-53 (2016)
PMID: 26894672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.48 KDa
Source organism: Influenza A virus (A/swine/Quebec/1257777/2010(H3N2))
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G3LZH2 (Residues: 108-270; Coverage: 30%)
Gene name: PB2
Sequence domains: Influenza RNA polymerase PB2 CAP binding domain

Ligands and Environments

1 bound ligand:
Ligand SCN 8 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 54.611Å b: 54.611Å c: 196.486Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.124 0.122 0.177
Expression system: Escherichia coli BL21

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Citations

4 review citations

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design.
Stevaert et al. (2016)
3 more