› 5eii

X-ray diffraction
2.44Å resolution

Structural determination of an protein complex of a Fab with increased solubility

Released:
DOI: 10.2210/pdb5eii/pdb
Model geometry
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PDB entry 5eii coloured by chain, front view.
PDB entry 5eii coloured by chain, side view.
PDB entry 5eii coloured by chain, top view.
Source organisms:
Entry authors: Bailey LJ, Kossiakoff AA, Chaperone-Enabled Studies of Epigenetic Regulation Enzymes (CEBS)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211067 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 235 amino acids
Theoretical weight: 25.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Histone chaperone ASF1 Chains: G, I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 81.486Å b: 62.945Å c: 161.828Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli

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