› 5ekg

X-ray diffraction
2.8Å resolution

Crystallization and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a XPG NLS Peptide, fragment 2

Released:
DOI: 10.2210/pdb5ekg/pdb
PDB entry 5ekg coloured by chain, front view.
PDB entry 5ekg coloured by chain, side view.
PDB entry 5ekg coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Calorimetric Studies Demonstrate that Xeroderma Pigmentosum Type G (XPG) Can Be Imported to the Nucleus by a Classical Nuclear Import Pathway via a Monopartite NLS Sequence.
Barros AC, Takeda AA, Dreyer TR, Velazquez-Campoy A, Kobe B, Fontes MR
J Mol Biol 428 2120-31 (2016)
PMID: 26812207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151271 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA excision repair protein ERCC-5 Chains: B, C
Molecule details ›
Chains: B, C
Length: 19 amino acids
Theoretical weight: 2.41 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P28715 (Residues: 1168-1186; Coverage: 2%)
Gene names: ERCC5, ERCM2, XPG, XPGC
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 78.492Å b: 90.842Å c: 100.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.228
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

6 citation in other articles

Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms.
Wu et al. (2017)
5 more

3 mentions without citation

Extreme Dynamics in the BamA β-Barrel Seam.
Doerner et al. (2017)
2 more