5ekq

X-ray diffraction
3.39Å resolution

The structure of the BamACDE subcomplex from E. coli

Released:
Source organism: Escherichia coli K-12
Primary publication:
The structure of the β-barrel assembly machinery complex.
Science 351 180-6 (2016)
PMID: 26744406

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-141765 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 790 amino acids
Theoretical weight: 88.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 21-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 25.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC02 (Residues: 20-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Structure domains: Tetratricopeptide repeat domain
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A903 (Residues: 25-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A937 (Residues: 20-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Structure domains: Beta-lactamase Inhibitory Protein; Chain:B, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 234.848Å b: 109.231Å c: 103.988Å
α: 90° β: 95.04° γ: 90°
R-values:
R R work R free
0.24 0.238 0.281
Expression system: Escherichia coli BL21(DE3)