› 5elb

X-ray diffraction
1.08Å resolution

Cholera toxin classical B-pentamer in complex with Lewis-y

Released:
DOI: 10.2210/pdb5elb/pdb
PDB entry 5elb coloured by chain, front view.
PDB entry 5elb coloured by chain, side view.
PDB entry 5elb coloured by chain, top view.
Source organism: Vibrio cholerae O1
Primary publication:
High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Heggelund JE, Burschowsky D, Bjørnestad VA, Hodnik V, Anderluh G, Krengel U
PLoS Pathog 12 e1005567 (2016)
PMID: 27082955

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cholera enterotoxin B-subunit Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Vibrio cholerae O1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57193 (Residues: 22-124; Coverage: 100%)
Gene names: ERS013165_03981, ctxB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, NDG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, NDG, FUC, NAG
Ligand GAL 2 x GAL
Ligand NDG 2 x NDG
Ligand FUC 4 x FUC
Ligand NAG 2 x NAG
5 bound ligands:
Ligand CA 10 x CA
Ligand GAL 10 x GAL
Ligand GLA 10 x GLA
Ligand BCN 10 x BCN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 72.333Å b: 98.984Å c: 152.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.12 0.155
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
6 more

8 mentions without citation

High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Heggelund et al. (2016)
7 more