› 5ema

X-ray diffraction
1.32Å resolution

Crystal structure of the SNX27 PDZ domain bound to the phosphorylated C-terminal LRRC3B PDZ binding motif

Released:
DOI: 10.2210/pdb5ema/pdb
PDB entry 5ema coloured by chain, front view.
PDB entry 5ema coloured by chain, side view.
PDB entry 5ema coloured by chain, top view.
Source organisms:
Primary publication:
A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex.
Clairfeuille T, Mas C, Chan AS, Yang Z, Tello-Lafoz M, Chandra M, Widagdo J, Kerr MC, Paul B, Mérida I, Teasdale RD, Pavlos NJ, Anggono V, Collins BM
Nat Struct Mol Biol 23 921-932 (2016)
PMID: 27595347

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185088 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sorting nexin-27 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 10.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K4V4 (Residues: 39-133; Coverage: 18%)
Gene names: Mrt1, Snx27
Sequence domains: PDZ domain
Leucine-rich repeat-containing protein 3B Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 925 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96PB8 (Residues: 252-259; Coverage: 4%)
Gene names: LRP15, LRRC3B, UNQ195/PRO221

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 37.171Å b: 48.49Å c: 56.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.179
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

To degrade or not to degrade: mechanisms and significance of endocytic recycling.
Cullen et al. (2018)
30 more