› 5en5

X-ray diffraction
2.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151939 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 609 amino acids
Theoretical weight: 65.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 39-329, 561-869; Coverage: 57%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
DARPin Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: Ribosome display vector pRDV
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 108.341Å b: 145.286Å c: 174.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.249
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
30 more

2 mentions without citation

Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives.
Sjuts et al. (2016)
1 more