› 5ep4

X-ray diffraction
1.5Å resolution

Structure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO

Released:
DOI: 10.2210/pdb5ep4/pdb
PDB entry 5ep4 coloured by chain, front view.
PDB entry 5ep4 coloured by chain, side view.
PDB entry 5ep4 coloured by chain, top view.
Source organism: Photobacterium angustum
Primary publication:
Structure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO.
Boyaci H, Shah T, Hurley A, Kokona B, Li Z, Ventocilla C, Jeffrey PD, Semmelhack MF, Fairman R, Bassler BL, Hughson FM
PLoS Biol 14 e1002464 (2016)
PMID: 27219477

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative repressor protein luxO Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.61 KDa
Source organism: Photobacterium angustum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1ZS18 (Residues: 140-387; Coverage: 52%)
Gene name: VAS14_05548
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand EPE 1 x EPE
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P61
Unit cell:
a: 73.69Å b: 73.69Å c: 81.792Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.155 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Bacterial Enhancer Binding Proteins-AAA+ Proteins in Transcription Activation.
Gao et al. (2020)
4 more

1 mention without citation

Protein Model and Function Analysis in Quorum-Sensing Pathway of Vibrio qinghaiensis sp.-Q67.
Wang et al. (2021)