› 5eq2

X-ray diffraction
1.8Å resolution

Crystal Structure of the SrpA Adhesin from Streptococcus sanguinis

Released:
DOI: 10.2210/pdb5eq2/pdb
PDB entry 5eq2 coloured by chain, front view.
PDB entry 5eq2 coloured by chain, side view.
PDB entry 5eq2 coloured by chain, top view.
Source organism: Streptococcus sanguinis SK36
Primary publication:
Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin.
Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM
J Biol Chem 291 7230-40 (2016)
PMID: 26833566

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.05 KDa
Source organism: Streptococcus sanguinis SK36
Expression system: Escherichia coli
UniProt:
  • Canonical: A3CM52 (Residues: 249-449; Coverage: 12%)
Gene names: SSA_0829, srpA
Sequence domains:
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:
Ligand CA 7 x CA
Ligand ACT 8 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F, APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 174.522Å b: 46.769Å c: 64.761Å
α: 90° β: 102.72° γ: 90°
R-values:
R R work R free
0.176 0.175 0.207
Expression system: Escherichia coli

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Citations

3 review citations

Streptococcus sanguinis biofilm formation & interaction with oral pathogens.
Zhu et al. (2018)
2 more

6 mentions without citation

Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin.
Bensing et al. (2016)
5 more