› 5es4

X-ray diffraction
3.3Å resolution

RE-REFINEMENT OF INTEGRIN ALPHAXBETA2 ECTODOMAIN IN THE CLOSED/BENT CONFORMATION

Released:
DOI: 10.2210/pdb5es4/pdb
PDB entry 5es4 coloured by chain, front view.
PDB entry 5es4 coloured by chain, side view.
PDB entry 5es4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Leukocyte integrin αLβ2 headpiece structures: The αI domain, the pocket for the internal ligand, and concerted movements of its loops.
Sen M, Springer TA
Proc Natl Acad Sci U S A 113 2940-5 (2016)
PMID: 26936951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138498 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (14 distinct):
Integrin alpha-X Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 1137 amino acids
Theoretical weight: 125.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P20702 (Residues: 20-1103; Coverage: 95%)
Gene names: CD11C, ITGAX
Sequence domains:
Structure domains:
Integrin beta-2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 727 amino acids
Theoretical weight: 80.63 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P05107 (Residues: 23-696; Coverage: 90%)
Gene names: CD18, ITGB2, MFI7
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand CA 16 x CA
Ligand MG 1 x MG
Ligand NAG 27 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 132.03Å b: 163.48Å c: 536.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.257 0.307
Expression system: Cricetulus griseus

Quick links

Citations

4 review citations

Research advances on structure and biological functions of integrins.
Pan et al. (2016)
3 more

13 mentions without citation

Structural Immunology of Complement Receptors 3 and 4.
Vorup-Jensen et al. (2018)
12 more